Table 1.

Primers for PCR amplification

Gene	GenBank	Primers sequences	T m (°C)
GAPDH	NM_00246	Forward 5′‐TAACTCTGGTAAAGTGGATATTG‐3′ Reverse 5′‐GAAGATGGTGATGGGATTTC‐3′	58
COL1	NM_000088	Forward 5′‐GGAATGAGGAGACTCGCAACC‐3′ Reverse 5′‐TCAGCACCACCGATGTCCAAA‐3′	58
BMP2	NM_001200	Forward 5′‐ATGAAGAATCTTTGGAAGAACTAC‐3′ Reverse 5′‐GGTGATGGAAACTGCTATTG‐3′	58
vWF	NM_000552	Forward 5′‐AAGAAAATAACACAGGTGAA‐3′ Reverse 5′‐TACTCTCCTCTCTCATTGAC‐3′	58
ALP	NM_001177530	Forward 5′‐AGCCCTTCACTGCCATCCTGT‐3′ Reverse 5′‐ATTCTCTCGTTCACCGCCCAC‐3′	58
RUNX2	NM_001024630	Forward 5′‐GGGTAACGATGAAAATTATTCT‐3′ Reverse 5′‐TTAATTGCTCTGTGATAGGTA‐3′	58
ANGPT1	NM_001199859	Forward 5′‐ACCGAGCCTATTCACAGTAT‐3′ Reverse 5′‐ACAGTTGTCATTATCAGCATCTT‐3′	58
ANGPT2	NM_001118888	Forward 5′‐GTGATTAGACAGAACACCTATGC‐3′ Reverse 5′‐AACAGTGCTCAGAAGAATGC‐3′	58
VE‐cadherin	NM_001795	Forward 5′‐GCAATAGACAAGGACATAACA‐3′ Reverse 5′‐TAGGAAGTGGACCTTGGTAT‐3′	58
VEGF‐165	AB021221	Forward 5′‐TATGCGGATCAAACCTCACCA‐3′ Reverse 5′‐CACAGGGATTTTTCTTGTCTTGCT‐3′	58