Figure 6.

G6PD, HMGCoA reductase and LDL receptor mRNA levels during the growth of mononuclear cells from G6PD‐normal and ‐deficient subjects. Freshly isolated PBMC from G6PD‐normal and ‐deficient subjects were cultured at a concentration of 1 × 10⁶cells/ml in RPMI medium supplemented with 10% FCS. Unstimulated cells (0 h) and cells incubated with PHA (5 µg/ml) were harvested 3, 8, 16, 24, 48 and 72 h after addition. At the times indicated, cells were harvested, total RNA was extracted and RT‐PCR performed as described in Materials and Methods. The lower panel represents the densitometric quantification of mRNA levels expressed as the ratio of target gene to L7 mRNA levels. Similar results were obtained in three distinct experiments performed using different subjects.