Table 1.
Primer sequences and size of RT‐PCR products
Gene | Primers RT‐PCR | RT‐PCR protocol | Size of RT‐PCR products (number of cycles) |
---|---|---|---|
HMG‐CoA | 5′TACCATGTCAGGGGTACGTC 3′ | 95 °C, 60 s/60 °C, 45 s/ | 246 |
reductase | 5′CAAGCCTAGAGACATAATCATC 3′ | 72 °C, 45 s (35) | |
LDL receptor | 5′CAATGTCTCACCAAGCTCTG 3′ | 95 °C, 60 s/60 °C, 45 s/ | 258 |
5′TCTGTCTCGAGGGGTACCTG 3′ | 72 °C, 45 s (35) | ||
G6PD | 5′CTGGAACCGCATCATCGTG 3′ | 94 °C, 50 s/60 °C, 50 s/ | 916 |
5′TTCTCATCACGGACGTCATCT 3′ | 68 °C, 90 s (25) | ||
L7 | 5′CCTGAGGAAGAAGTTTGCCC 3′ | 95 °C, 30 s/60 °C, 60 s/ | 286 |
5′CTTGTTGAGCTTCACAAAGGTGCC 3′ | 72 °C, 60 s (25) |