Cell cycle distribution and expression of cell cycle regulators in
freshly isolated transgenic and nontransgenic control thymocytes.
(Left) Cell cycle distribution of freshly isolated
thymocytes from nontransgenic, MyrAkt, and AktE40K transgenic mice.
There were four 6- to 8-week-old, age- and sex-matched mice per group.
(Right) Expression of cell cycle regulators in freshly
isolated thymocytes from nontransgenic control and transgenic mouse
lines. Lanes: 1, nontransgenic; 2, MyrAkt1 M-1; 3, MyrAkt1 M-9; 4,
Akt1-E40K E-3; and 5, Akt1-E40K E-4. For cyclin D3 and cyclin D2, the
results of two independent experiments are shown. The same blots were
probed with the antibodies to cyclin D2 and cyclin D3. The intensity of
individual bands was measured by densitometry. Band intensity ratios
were as follows: cyclin D3, nontransgenic vs. MyrAkt M-1 transgenic
thymocytes = 2.23 and 1.86 in the two experiments shown; cyclin
D2, nontransgenic vs. MyrAkt M-1 transgenic thymocytes = 0.42 and
0.45; cyclin E, nontransgenic vs. MyrAkt M-1 transgenic thymocytes
= 0.25; cdk2, nontransgenic vs. MyrAkt M-1 transgenic thymocytes =
2.89.