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Primary thymomas from MyrAkt transgenic mice express high levels of cyclin D3. (A) Western blots of cell lysates from freshly isolated and cultured MyrAkt transgenic thymocytes, as well as from freshly isolated tumor cells from six thymomas (three in each of two separate experiments) derived from MyrAkt transgenic mice were probed with an antibody to cyclin D3. (B) Equal loading was confirmed in the second experiment by reprobing with an anti β-actin antibody (Sigma).
