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. 2008 Jan 16;41(1):122–135. doi: 10.1111/j.1365-2184.2007.00506.x

Figure 2.

Figure 2

Figure 2

Effects of cell density and cell cycle progression on PKC‐ι concentration in T98G and U‐138MG glioma cells. FACS analysis of DNA content in 100% confluent T98G (a) or U‐138MG cells (c) and 50% confluent T98G (b) or U‐138 MG (d) cells. U‐138 glioma cells are aneuploid; U‐138MG DNA histograms are from one representative experiment and illustrate two cycling populations with a G0/G1 peak at 2 N (first red shaded peak) and another at 4 N (second yellow unshaded peak). Total DNA content for G0/G1, DNA synthetic phase (S), gap 2 and mitosis (G2/M) was quantified by addition of each of the phases in both populations. Forty thousand events were collected per time point and treatment group. Western blots of PKC‐α, PKC‐β1, PKC‐δ, PKC‐ɛ, PKC‐ι, and anticaspase 7 present in T98G or U‐138MG cells that were at different stages of confluence (e). Band intensity of PKC‐ι in T98G (f) and U‐138 MG (g) was quantified by densitometry.