Table 2.

 Oligonucleotide sequences used for RT‐PCR analysis

Markers	Sequences (5′→3′)	Product size (bp)	Annealing temperature (°C)
Glyceraidehyde‐3‐phosphate dehydrogenase (GAPDH)	S: AGATCAAGAAGGTGGTGAAG A: TTGTCATACCAGGAAATGAGC	178	60
Endoglin (CD105)	S: AAGAGCTCATCTCGAGTCTG A: TGACGACCACCTCATTACTG	162	56
CD34 molecule (CD34)	S: CAGAAATTCCCAGCAAGCTC A: ATAGCAAATGAGGCCCAAGA	207	56
Integrin β‐1 (CD29)	S: CTTATTGGCCTTGCATTGCT A: TTCCCTCGTACTTCGGATTG	184	63
CD44 antigen (CD44)	S: ATCCTCACGTCCAACACCTC A: CTCGCCTTTCTTGGTGTAGC	165	63
ALCAM (CD166)	S: CCGTTCACTATTTGGATTTGT A: CGTTTCACAGACATAGTTTCC	199	55
CD14 molecule (CD14)	S: TTGATCTCAGCTGCAACAGG A: GTGGGGATATCAGGAACCCT	184	56
Major histocompatibility complex I (MHC‐I)	S: GGAGAGGAGCAGAGATACA A: CTGTCACTGTTTGCAGTCT	218	55
Major histocompatibility complex II (MHC‐II)	S: TCTACACCTGCCAAGTG A: CCACCATGCCCTTTCTG	178	55
Osteocalcin (BGLAP)	S: TGAAGACCAGTATCCTGATGC A: GCTGACTTGTTTCCTGACTG	174	60
Osteopontin (OPN)	S: GTCTGGCAGAGGTGCAGCCT A: ATGTGGTCAGCCAGCTCGTC	173	56
Peroxisome Proliferator‐activated Receptor (PPAR‐γ)	S: TGCCCTTCAACGAAATTACC A: TGGAATGTCTTCATAGTGTGG	176	55
Adiponectin (ADIPQ)	S: GGAGACAGCTACTCCCCAAGAT A: GTCCAGTCTTACCTCTCAAACCT	187	64
Aggrecan (ACAN)	S: TCTGCTACACAGGTGAAGAC A: AAGATGGGTTTCACTGTGAG	174	60
Collagen type 2, alpha I (COL2A1)	S: GGAGACTACTGGATTGACCC A: TCCATAGCTGAAGTGGAAGC	201	60
Glial Fibrillary Acidic Protein (GFAP)	S: CAGAAGCTCCAGGATGAAACC A: TGGATCTTCCTCAAGAACCGGA	155	58
Nestin (NES)	S: TGCCCTCAGCTTGCAGGAC A: GTGTCTCGAGAGTATCAGGCAAG	134	60

S, sense; A, antisense; bp, base pairs.