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Figure 2.

Figure 2

Effects of PD 406976 on U‐373MG cell cycle distribution as a function of time. FACS analysis of DNA replication during a mitotic time course. Cells were stimulated with 10% FCS and either 5.5 µl of DMSO/7 ml of media (controls; left column) or PD 406976 (2 µm; right column). To establish DNA flow cytometry settings, U‐373MG cells were karyotyped (Genzyme Genetics, Tampa, FL, USA) and found to be aneuploid. The number of events collected was 40 000 per time point and treatment group. Data are representative of three experiments.