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. 2005 Apr 14;38(2):87–106. doi: 10.1111/j.1365-2184.2005.00333.x

Figure 8.

Figure 8

Western blot analysis of PKC isoenzyme expression in (a) SVG and (b) U‐373MG cells post‐PD 406976 treatment. Equal amounts of cellular protein (10 µg) were loaded per well and antibodies (used at 1 : 2000 dilutions; 0.1 µg/ml) against the antigens which were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) for PKC‐α (SC‐8393), PKC‐βI (SC‐8049), PKC‐γ (SC‐211), PKC‐δ (SC‐937), aPKC‐ζ (SC‐216), and actinin (SC‐1616) and from BD Transduction Laboratories (Lexington, KY, USA) for PKC‐τ (catalogue no. 610176). Secondary antibodies were obtained from Accurate (Westbury, NY, USA) (JGM035146, JGM025144) and were used at 1 : 15 000 dilution. Representative immunoblots from three independent experiments are shown.