Figure 10.

Zerumbone inhibited the proliferation of HeLa cells in synergism with vinblastine and paclitaxel. HeLa cells were treated with different concentrations of vinblastine (A) and paclitaxel (B) for 24 h, and the inhibition of cell proliferation was determined using SRB assay as described in Section 2. Median effect plot for the inhibition of cell proliferation by vinblastine (C), paclitaxel (D) and zerumbone (E). Zerumbone synergistically inhibited the proliferation of HeLa cells in combination with vinblastine (F) and paclitaxel (G). The combined effect of zerumbone with vinblastine (H) and paclitaxel (I) on the proliferation of HeLa cells was calculated using the Chou and Talalay equation, and the CI at different drug concentrations was calculated. All the experiments were performed three independent times, and the error bars represent mean ± SD