Figure 2.

Adipogenic, osteogenic and chondrogenic differentiation of human UC ‐ MSC s and BM ‐ MSC s. UC‐MSCs (a) and BM‐MSCs (b) were examined for their multipotency. Cells were cultured in different differentiation medium for 21 days or in growth medium alone as control. Adipogenic differentiation was detected by formation of neutral lipid droplets stained with oil red O 1, 2. Osteogenic differentiation was examined using von Kossa staining, for deposition of mineralized matrix 3, 4 and ALP staining for alkaline phosphatase activity 5, 6. Chondrogenic differentiation was analysed using alcian blue staining for extracellular matrix glycosaminoglycans 7, 8.