Table 2.
The effect of a single dose of rmIL‐17 on cytokine release and nitric oxide (NO) production by bone marrow cells
| Time | Group | IL‐6 (pg/ml) | IL‐10 (pg/ml) | IFN‐γ (pg/ml) | IGF‐I (pg/ml) | NO (µmol) |
|---|---|---|---|---|---|---|
| N | 187.9 ± 31.7 | 52.55 ± 4.9 | < 2‡ | 3.62 ± 0.7 | 2.75 ± 0.3 | |
| 6 h | IL‐17 | 470.75 ± 41.3***, † | 121.12 ± 5.9*** | < 2 | 2.2 ± 0.1 | 4.94 ± 0.8**, † |
| Saline | 310.25 ± 16.7* | 142.41 ± 20.6*** | < 2 | 3.23 ± 0.8 | 3.0 ± 0.4 | |
| 24 h | IL‐17 | 204.4 ± 39.9 | 78.6 ± 13.1 | < 2 | 3.77 ± 0.8 | 3.8 ± 0.6 |
| Saline | 192.83 ± 65.0 | 79.5 ± 7.7 | < 2 | 2.15 ± 0.2 | 2.9 ± 0.8 |
The cells were obtained from non‐treated mice (N) and IL‐17‐ and saline‐treated 6 h and 24 h after injection. Cells (5 × 106/ml) were cultured for 48 h at 37 °C and cell‐free supernatants were collected. Values are expressed as means ± SEM. Significance at *P < 0.05, **P < 0.01, ***P < 0.001 for IL‐17‐ and saline‐treated vs. non‐treated mice, and at †P < 0.05 for IL‐17‐treated vs. saline‐treated mice. ‡The secretion of IFNγ was below detectable levels.