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Percentage of insulin‐positive cells as in the order of 50% and C‐peptide‐positive cells in the final induction stage were TUNEL‐negative. (a) Immunocytochemical analysis of insulin. (b) Statistics of insulin‐positive cells in the final induction stage. (c–e) As a positive control, differentiated skin‐derived precursors were treated with DNase to induce apoptosis and stained with TUNEL. (f–i) Double staining C‐peptide and TUNEL of insulin‐producing cells in Stage 3. Scale bar: 50 µm.
