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. 2009 Jan 6;42(1):49–62. doi: 10.1111/j.1365-2184.2008.00573.x

Figure 5.

Figure 5

Insulin‐producing cells (IPCs) in Stage 3 express characteristic pancreatic β‐cell markers and did not express markers of differentiated neural cells. (a) Co‐staining of Stage 3 IPCs with antibodies against insulin and other pancreatic endocrine hormones showing that insulin‐positive cells also expressed C‐peptide but not glucagon, somatostatin or amylase. (b) Western blot analysis to confirm the expression of endocrine hormones. (c) Co‐expression of insulin and the indicated marker genes specific for the endocrine β‐cells (Isl‐1, Nkx6.1, Glut‐2 and Pdx‐1) in IPCs. (d) No co‐staining of C‐peptide or neural lineage markers (β‐III tubulin, MAP2 and GFAP) was found in Stage 3 IPCs. Scale bar: 50 µm.