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. 2007 Sep 14;40(5):768–779. doi: 10.1111/j.1365-2184.2007.00459.x

Figure 2.

Figure 2

(a) Effects of NS‐398 on cell migration of HT29, HCA7 and HCT116 human colonic adenocarcinoma cell lines. Cells were treated with 5 and 10 µm NS‐398 for 24 h. 1 × 105 cells were resuspended in serum‐free and calcium‐free (SFCF) medium and were added to each insert filter. SFCF medium was placed in wells. After 24 h, the number of migrated cells in 10 high power fields was counted. Data represent mean ± SE of three independent experiments done in triplicates. ***P < 0.001 compared to control. (b) Effects of PGE2 on motility of colon cancer cells. (a) PGE2 stimulated cell migration in these human colonic carcinoma cells. 1 × 105 cells resuspended in SFCF were seeded into the insert filter, and the assay was carried out for 24 h with either vehicle or 1 µm PGE2 as attractant. (b) PGE2 partially reversed effect of NS‐398 on cell migration. 1 µm PGE2 was added to each insert containing 1 × 105 NS‐398‐treated cells. SFCF was placed in the wells. After 24 h the number of migrated cells in 10 high power fields was counted. Data represent mean ± SE of three independent experiments performed in triplicates. ***P < 0.001 control versus PGE2 and NS‐398 versus NS‐398+PGE2. (c) EP receptor expression in colon cancer cells. EP receptor mRNA expression in human HT29, HCA7 and HCT116 human colonic adenocarcinoma cells detected by RT‐PCR. No bands were observed in RNA samples that had not undergone reverse transcription.