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. 2015 Oct 21;48(6):631–642. doi: 10.1111/cpr.12227

Figure 5.

Figure 5

Knockdown of Tbx2 induced G1 cell cycle arrest and regulated expression of cell cycle‐related proteins. (a) Representative flow cytometric analysis of melan‐a cells either mock or transfected with si‐C, si‐Tbx2‐1 or si‐Tbx2‐2. Results show that percentages of cells in G1 phase increased after knockdown of Tbx2. (b) Quantification (mean ± SD) from triplicate flow cytometric analyses of percentages of G1, G2/M and S cell populations in melan‐a cell transfected with si‐C, si‐Tbx2‐1 or si‐Tbx2‐2. *P < 0.05. (c) Western blot analysis of cell cycle‐related proteins in melan‐a cells and melan‐a cells transfected with si‐C, si‐Tbx2‐1 or si‐Tbx2‐2. Note that besides Cyclin D1, several other G1‐phase related proteins, such as CDK4, pRB, and E2F1, were reduced after knockdown of Tbx2. Intensity of bands was measured using ImageJ software and fold‐change is given in comparison to intensities in mock cells.