Table 1.
Equations to establish the timing of each phase of the cycle
| Cell cycle parameter | Equations |
|---|---|
| Sl | S lu + G 2 lu + ½G 1 ld + ½S ld |
| TS | (S l/(G 2 lu + ½(G 1 ld + S ld)) × t |
| TG2 | (½(G 1 ld + S ld)/S l) × t |
| TG1 | t × T G2 − (½S ld/S l) × T S |
| TC | T G1 + T S + T G2 |
| GF (%) | (LI/LIP) × 100 |
| LIP | (T S/T C) × T S |
| LI (%) | (S l/n t − ½(G 1 ld + S ld) − (G 2 − G 2 lu)) × 100 |
G 1 ld, labelled divided G1 cells; G 2 lu, labelled undivided G2 cells; GF, growth fraction; LI, labelling index of the whole population; LIP, labelling index of proliferating cells; n t, total number of BrdUrd positive and negative cells per sample; S l, labelled S cells; S lu, labelled undivided S cells; t, time of BrdUrd incorporation; T, time of phase cycle.