Figure 1.

Dependence of the proliferative life span of EAhy926 cells on seeding density. Endothelial EAhy926 cells from the same subpopulation were plated at the seeding density of 15.10³ cells/cm² (Fig. 1a) and 30.10³ cells/cm² (Fig. 1b). Data are expressed as mean ± SD of cell densities counted in three different wells. Solid lines indicate the corresponding fit of the ND model to each set of data, considered separately. These curves were superimposed in Fig. 1c. The corresponding simulated evolution of the nutrient concentration of the cell culture medium is indicated in Fig. 1d, with the upper and thicker curve corresponding to the plating density of 15.10³ cells/cm². The culture medium remained the same in these experiments (J0 assay).