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. 2013 Aug 24;46(5):595–605. doi: 10.1111/cpr.12056

Figure 1.

Figure 1

Effects of salvianolic acid A ( SAA ) on lipopolysaccharide ( LPS )‐induced production of nitric oxide and viability of RAW264.7 cells. Cells were treated with LPS (1 μg/ml) alone or LPS plus different concentrations of SAA (125, 250 and 500 μm) for 24 h. Amounts of nitric oxide were quantified using Griess reagent (a) and cell viability was measured by MTT assay (b). Cell morphological changes were observed using phase contrast microscopy (c: medium; d: 500 μm SAA; e: 1 μg/ml LPS; f: 125 μm SAA plus 1 μg/ml LPS; g: 250 μm SAA plus 1 μg/ml LPS; h: 500 μm SAA plus 1 μg/ml LPS, c–h ×400 magnification). Data are presented as mean ± SD of three independent experiments, a P < 0.01 versus LPS group. Con, control; Bar, 10 μm.