Figure 3.

Examples of the engraftment of haematopoietic lineage marrow cells (HLMCs) and mesenchymal stem cells (MSCs) in the bone marrow (BM) and spleen of irradiated mice transplanted with female GFP⁺ HLMCs and male GFP MSCs. HLMCs (brown colour) were detected in the BM (a, ×400) and spleen (b, ×200), demonstrated by the immunohistochemical method. MSCs are detected via direct FISH (c and e, ×1000) and indirect FISH (g and i, ×400). White arrows point to Y chromosome (Y chromosome paint seen as a green nuclear signal) under direct FISH and black arrows point to Y chromosomes (Y chromosome paint seen as a brown nuclear signal) under indirect FISH. (d), (f), (h) and (j) are the higher magnification (×600) of yellow boxes in the (c), (e), (g) and (i), respectively. BMT, bone marrow transplantation; FISH, fluorescence in situ hybridization.