Skip to main content
. 2009 Jan 8;42(1):94–109. doi: 10.1111/j.1365-2184.2008.00576.x

Figure 10.

Figure 10

Apoptosis in siRNA c‐myc cells. Control and siRNA c‐myc cells evaluated at 48 and 72 h. The sub‐G1 peak (arrows) represents the percentage of apoptosis. (b) TUNEL assay. Percentages of apoptotic cells in control (black) and siRNA c‐myc (white) at 48 and 72 h. Statistical significance between the different groups was evaluated by Student's t‐test (**P < 0.001; *P < 0.01). A P‐value < 0.001 was considered significant. (c) Western blot analysis of c‐IAP1 and c‐IAP2 levels with or without proteasome inhibitor MG132. Control and siRNA c‐myc cells were treated or not treated with 0.25 µm proteasome inhibitor MG132 for 4 h 24 h after the trasfection. Protein levels were quantified by densitometry (TotalLab image analysis solution, version 2003) and normalized for β‐actin. The experiment was repeated three times showing similar results.