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. 2009 Jan 8;42(1):94–109. doi: 10.1111/j.1365-2184.2008.00576.x

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© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd

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Determination of the optimal schedule of hormone treatment in both T98G and ADF pINDc‐myc As clones. Cell growth number effects in both T98G and ADF clones exposed to 5 (), 10 (), 15 (), 20 (*) and 25 µm () dosages of ponasterone given every 24 h. As control we used pINDneo () for each cell line. At the indicated times, from day 2 (24 h after induction) to day 5 (96 h after induction), cells were harvested and counted by using trypan blue dye exclusion. All data are expressed as means ± standard deviation of three independent experiments with similar results. Statistical significance of the data was evaluated by one‐way analysis of variance followed by Tukey post‐hoc test.

Inline graphic — Determination of the optimal schedule of hormone treatment in both T98G and ADF pINDc‐myc As clones. Cell growth number effects in both T98G and ADF clones exposed to 5 (), 10 (), 15 (), 20 (*) and 25 µm () dosages of ponasterone given every 24 h. As control we used pINDneo () for each cell line. At the indicated times, from day 2 (24 h after induction) to day 5 (96 h after induction), cells were harvested and counted by using trypan blue dye exclusion. All data are expressed as means ± standard deviation of three independent experiments with similar results. Statistical significance of the data was evaluated by one‐way analysis of variance followed by Tukey post‐hoc test.