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. 2009 Jan 8;42(1):94–109. doi: 10.1111/j.1365-2184.2008.00576.x

Figure 4.

Figure 4

Cell cycle analysis after continuous bromodeoxyuridine (BrdUrd) incorporation in ADF pINDneo control and ADF pINDc‐myc As clones. The dosage of 10 µm BrdUrd was added continuously to the medium for 48 h starting from 24 h of hormone induction (dosage 20 µm given every 24 h). The regions represent BrdUrd‐negative G1 cells. Samples were acquired using a FACScan cytofluorimeter (Becton Dickinson). Biparametric flow cytometry analysis was performed using the CellQuest software package. The experiment was repeated three times showing similar results.