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. 2006 Nov 15;39(6):599–609. doi: 10.1111/j.1365-2184.2006.00402.x

Figure 4.

Figure 4

Effect of syringolin A on procaspase‐3 and Akt/PKB protein levels in human neuroblastoma cells. SK‐N‐SH cells were cultured in the absence or presence of 20 µm syringolin A. Cells were harvested after 0, 24 and 48 h, lysed, and cell lysates probed for (a) procaspase‐3 and (b) protein kinase Akt/PKB by Western blot analysis. Both procaspase‐3 and Akt/PKB protein levels and also β‐actin protein levels decreased in syringolin A‐treated cells after 48 h, thus suggesting a general shutdown of protein synthesis in these cells. (c) The Coomassie‐stained membrane depicts total amounts of protein in each lane and the black arrow indicates a prominent protein band at approximately 72 kDa. Equal amounts of total protein were loaded in all experiments (10 µg per lane) as judged by protein assay using the protein dye reagent from Bio‐Rad. The data are representative of 3 (procaspase‐3) or 4 (Akt/PKB) independent experiments.