Figure 4.
Thyrotroph embryonic factor (TEF) is involved in the cell cycle G1/S transition. (A) GSEA plots of published TCGA data demonstrated a positive correlation between TEF mRNA expression and cell cycle check point signatures, especially in the G1/S phase. (B) Flow cytometric analysis of stable cell lines constructed from RT4 and HT‐1376 cells demonstrated that the percentage of S phase cells negatively correlated with the expression of TEF. (C and D) Representative micrographs (C) and quantification (D) of BrdU incorporation in stable cell lines constructed from RT4 and HT‐1376 cells. Three independent experiments were conducted. (E) Western blot analysis of TEF and G1/S transition‐associated genes. (F) RT‐qPCR analysis of p21Cip1 and p27Kip1 expression in stable cell lines constructed from RT4 and HT‐1376 cells. *P < 0.05 for TEF vs Vector1; RNAi(s) vs Vector2. Data represent the mean ± SD