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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Photochem Photobiol. 2018 Nov 28;95(3):846–859. doi: 10.1111/php.13043

Figure 1.

Figure 1.

EC-specific retention of HPPH determined in reconstituted co-cultures of TEC-1 and CSFE-labeled lung (L364) tumor stromal cells. (A and B), Co-cultures were incubated in serum-free RPMI containing 3.2 μM HPPH (A) or HPPH-Lac (B) at 37°C for 30 min and then followed by a chase in fresh RPMI-10%FBS for 4 and 24h. The culture morphology was assessed by phase microscopy followed by imaging CSFE and PS fluorescence. (C) Comparison of cellular retention of HPPH, Foscan and Photofrin II. Co-cultures of TEC-1 and CSFE-stained stromal cells were first incubated for 4 h at 37°C in RPMI-10% FBS containing either 3.2 μM HPPH, 3.2 μM Foscan or 10 μg/ml Photofrin-II followed by a 24 h chase in PS-free medium. The culture and cell-associated PS were imaged by phase and fluorescent microscopy.