Figure 6.

Specificity of pheophorbide retention by human lung tumor cells. Primary cultures of lung T-ECs were grown to confluence in KSFM. A standard PS treatment was applied for every cell preparation: Uptake of the PSs indicated at the right was carried by incubating the cultures for 24 h in RPMI containing10%FBS and 1600 nM PSs followed by 24 h chase in PS-free medium. The cell-associated PS fluorescence was imaged by microscopy at 100X magnification using 2 sec exposures. All images were identically processed. Examples of PS retention patterns are shown. For comparison, the PS retention pattern of N-Fb is included at the left and the two most distinct patterns detected in cancer cells derived from PDX tissue (TEC-1–2 and TEC-21, still including murine stromal cells) are shown on the right.