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. Author manuscript; available in PMC: 2020 May 30.
Published in final edited form as: J Pharm Biomed Anal. 2019 Mar 5;169:133–141. doi: 10.1016/j.jpba.2019.03.006

Table 4.

Representative examples of chromatographic methods for the quantitation of Vitamin K in Cell Line or Cell Culture Media

Analytes Quantification
method		 Bio-matrix Bio
matrix
volume		 Internal
standard		 Linearity Limit of
detection		 References
MK-4, MK-5, MK-6 HPLC-UV LC-ESI-MS Flavobacterium 25 mL fermentati on liquor MK-4 N/A N/A (72)
Vitamin K3 (Menadione) LC-APCI-MS/MS Human urine, rat plasma, human cultured cell and media Urine: 0.5 mL Plasma: 0.5 mL Cultured cell and media: 1-5 mL MD-d8 Up to 50 ng/40 μL MD: 40 pg/40 μL MD-d3 and MD-d8: 2 pg/40 μL (70)
MK-8, ubiquinone-7, ubiquinone-8 LC-APCI-MS Escherichia coli 20-30 mg of dry cells Vitamin K1, ubiquinone-6, ubiquinone-10 N/A N/A (73)
Ubiquinones (UQ6) Menaquinones (MK-4) APCI-MS/MS APPI-MS/MS Escherichia coli N/A Isotopes of UQ6-10 MK-4 MK-4: <3 to 8999 fmol/μL for APPI <9.0 to 8999 fmol/μL for APCI MK-4: 2.2fmol/μ L UQ6: 1.7 fmol/μL (74)