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. Author manuscript; available in PMC: 2020 Apr 9.
Published in final edited form as: Biochemistry. 2019 Mar 27;58(14):1905–1917. doi: 10.1021/acs.biochem.8b01241

Figure 3. Comparison of SpyCas9WT and SpyCas92Pro activities using sgRNAdel on different DNA substrates at 5 mM Mg2+ ions.

Figure 3.

A) Sequences of DNA substrates (the sequence of non-complementary DNA strand is shown) used in this study. Bold and underlined sequences are mismatches in the protospacer while annealing to sgRNA. B) Graph shows the total activity with separate regions indicating the percentage of nicked (red shaded region) and linear products. The enzyme concentration was at 50 nM. For matched DNA and MM5 DNA, there are nine and six replications respectively, while for the rest there are three replications. Error bars represent SEM.