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. Author manuscript; available in PMC: 2020 Apr 9.
Published in final edited form as: Biochemistry. 2019 Mar 27;58(14):1905–1917. doi: 10.1021/acs.biochem.8b01241

Figure 6. Activity analysis of SpyCas9WT and SpyCas92Pro in HEK293T cells.

Figure 6.

A) TIDE analysis of cleavage by SpyCas9WT and SpyCas92Pro at different genomic loci. B) T7 endonuclease assay for DTS7 spacer (with 20 nt or 21 nt of length) and using shortened (del) or full length (FL) repeat-tracrRNA region. Black arrows indicate cleavage products produced by T7E1 on mismatches created as a result of Cas9 editing. C) Off-target activity of SpyCas9WT and SpyCas92Pro as measured by targeted deep sequencing, the unmodified controls show no editing.