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. Author manuscript; available in PMC: 2019 May 2.
Published in final edited form as: J Med Chem. 2018 Jun 11;61(12):5187–5198. doi: 10.1021/acs.jmedchem.8b00042

Figure 2. Time-of-addition approach to identify the targeted stage of M090.

Figure 2.

(A) Time course design. MDCK cells were infected with H1N1 (A/PR/8/34) at 100 TCID50. The virus absorption was indicated as −1 h and M090 was added at −3 to −1, −1 to 0, −1 to 2, 0 to 2, 2 to 4, 4 to 6, 6 to 8, 8 to 10 h post-infection. After each treatment period, M090 was removed and cells were incubated with culture medium till the end point. (B) M090 was added at each interval, and the virus yield was determined by measuring the TCID50. *p<0.05, ** p<0.01, compared to the condition without drug treatment.