FIGURE 2.

Genetic relationships and heterozygosity reduction curve in the strains of X. tropicalis. (A) Neighbor joining trees of the strains based on genetic distances (D_A) of 60 Simple Sequence Length Polymorphism (SSLP) markers (upper) and p-distance of mitochondrial haplotypes (2,328 bp; lower). Numbers on branches indicate percent bootstrap probability (only indicated > 50%). (B) Plot of expected heterozygosity (H_E) against generation number (t). The curved dotted strain indicates theoretical reduction of heterozygosity via single brother-sister matings for every generation, calculated from the following equation: H_E = H₀${\left(1-\frac{1}{2N_e}\right)}^t$.