Skip to main content
. 2018 Nov 5;25(2):43–60. doi: 10.1093/molehr/gay048

Figure 7.

Figure 7

Impact of RHOA inhibition, ROCK inhibition and actin filament disturbance on mRNA expression profiles. (A) Z-projection and optical section confocal images of actin filaments, visualized with phalloidin staining, in representative inhibitor-treated blastocysts for 8 h from E3.5 (Control: n = 10, RHOi/Rho inhibitor I: n = 14, Y27632: n = 14, Cytochalasin B: n = 14, Latrunculin A: n = 14). Scale bar = 50 μm. ROCK: RHO-associated coiled-coil containing kinase. (B and C) Quantitative RT-PCR analyses of E3.5 expanding blastocysts treated with no inhibitor (Con), RHO inhibitor I (Rho), and Y27632 (Y27; in B) or latrunculin A (Lat; in C). Asterisks indicate significant differences (P < 0.01; two-sample t-test) compared to the control. Error bars represent SD.