Figure 8.

Control of histone H2B monoubiquitylation with LINXa4. (a) Immunoblots demonstrating light-mediated control of Bre1, an E3 ligase, in a BRE1 deletion strain (bre1∆) by fusing it to wild type LINXa4, LINXa4_RLR, or LINXa4_RVH. Asterisks indicate nonspecific bands. (b) Quantifications of dark state band intensities from replicate western blots as in (a); (n = 3, mean ± s.e.m.; see Supplementary Figure 4 for controls and replicate blots). *P < 0.05 by unpaired two-tailed t-test.