Table 2. Crystallization.

	EcTet (31–252)	HiMon (33–253)
Method	Sitting-drop vapor diffusion	Hanging-drop vapor diffusion
Plate type	24-well sitting drop	Linbro (Hampton Research)
Temperature (K)	295	295
Protein concentration (mg ml−1)	19	10
Buffer composition of protein solution	150 mM NaCl, 25 mM Tris–HCl pH 7.5	0.1% β-mercaptoethanol, 1 mM EDTA, 0.1 mM benzamidine, 20 mM Tris–HCl pH 8.0
Composition of reservoir solution	0.03 M magnesium chloride hexahydrate, 0.03 M calcium chloride dihydrate, 5% glycerol, 10% PEG 20 000, 17% PEG monomethyl ether 550, 0.1 M Buffer System 1 pH 6.5†	20% polyethylene glycol (PEG) 10 000, 0.1 M HEPES pH 7.5
Volume and ratio of drop	2 µl, 1:1
Volume of reservoir	1 ml

^†Buffer System 1, 1.0 M stock solution: 30 ml 1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 3.1 was titrated with 24.1 ml 1 M imidazole pH 10.2 to a final pH of 6.5.