Table 1. Macromolecule-production information.
Source organism | Human |
DNA source | cDNA |
Forward primer | TAATACGACTCACTATAGGG |
Reverse primer | GCTAGTTATTGCTCAGCGG |
Cloning vector | pET-28a(+) |
Expression vector | pET-28a(+) |
Expression host | E. coli BL21-CodonPlus(DE3)-RIL |
Complete amino-acid sequence of the construct produced | |
SENP1 | APEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEIINFYMNMLMERKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQIPQQMNGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL |
SUMO2 | DHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG |