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. 2019 Apr 24;75(Pt 5):332–339. doi: 10.1107/S2053230X19004266

Table 1. Macromolecule-production information.

Source organism Human
DNA source cDNA
Forward primer TAATACGACTCACTATAGGG
Reverse primer GCTAGTTATTGCTCAGCGG
Cloning vector pET-28a(+)
Expression vector pET-28a(+)
Expression host E. coli BL21-CodonPlus(DE3)-RIL
Complete amino-acid sequence of the construct produced
 SENP1 APEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEIINFYMNMLMERKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQIPQQMNGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL
 SUMO2 DHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG