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. 2019 Apr 24;75(Pt 5):332–339. doi: 10.1107/S2053230X19004266

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well hanging-drop crystallization plate
Temperature (K) 293
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 50 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT, 5% glycerol, 0.02% NaN3
Composition of reservoir solution 20% PEG 6000, 0.1 M HEPES pH 7.5
Volume and ratio of drop 2 µl; 1:1
Volume of reservoir (µl) 500