Table 2. Crystallization.
| Method | Hanging-drop vapor diffusion |
| Plate type | 24-well hanging-drop crystallization plate |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 50 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT, 5% glycerol, 0.02% NaN3 |
| Composition of reservoir solution | 20% PEG 6000, 0.1 M HEPES pH 7.5 |
| Volume and ratio of drop | 2 µl; 1:1 |
| Volume of reservoir (µl) | 500 |