Table 1. Effects of enzymes and chemical solutions on some of the main antigens and proteins of the red blood cell membrane.
| Antigens/proteins | Enzymes/chemical solutions | |||||
|---|---|---|---|---|---|---|
| Papain | Bromelain | Trypsin | Alpha-chymotrypsin | Ficin | DTT | |
| A | + | + | + | + | + | 0 |
| B | + | + | + | + | + | 0 |
| H | + | + | + | + | + | 0 |
| D | + | + | + | + | + | 0 |
| C | + | + | + | + | + | 0 |
| C | + | + | + | + | + | 0 |
| E | + | + | + | + | + | 0 |
| E | + | + | + | + | + | 0 |
| K | 0 | 0 | 0* | 0* | 0 | - |
| K | 0 | 0 | 0* | 0* | 0 | - |
| Fya | - | - | 0 | - | - | 0 |
| Fyb | - | - | 0 | - | - | 0 |
| Fy6 | - | - | 0 | - | - | 0 |
| Fy3 | - | - | 0 | - | - | 0 |
| Jka | + | + | + | + | + | 0 |
| Jkb | + | + | + | + | + | 0 |
| Jk3 | + | + | + | + | + | 0 |
| Lea | + | + | + | + | + | 0 |
| Leb | + | + | + | + | + | 0 |
| M | - | - | - | 0 | - | 0 |
| N | - | - | 0 | -/0 | - | 0 |
| S | -/0 | - | 0 | - | -/0 | 0 |
| S | -/0 | - | 0 | - | -/0 | 0 |
| Dia | 0 | + | 0 | 0 | 0 | 0 |
| Dib | 0 | + | 0 | 0 | 0 | 0 |
| P | + | + | + | + | 0 | |
| I | + | + | + | + | + | 0 |
| Lua | 0 | 0 | - | - | 0 | W/- |
| Lub | 0 | 0 | - | - | 0 | W/- |
| Doa | + | + | - | W | + | - |
| Dob | + | + | - | W | + | - |
| Vel | 0 | 0 | 0 | 0 | 0 | 0/- |
Source: Antibody detection, identification, and compatibility testing — introduction. Method 3-11. Evaluating enzyme-treated red cells. In: Fung MK, Grossman BJ, Hillyer CD, Westhoff CM, editors. American Association of Blood Banks (AABB). Technical Manual. 19th ed. EUA: AABB; 2017;( 11 ) Girello AL, Kuhn TI. Pesquisa e identificação de anticorpos irregulares. In: Girello AL, Kuhn TI. Fundamentos da Imuno-hematologia eritrocitária. 3a ed. São Paulo: Senac; 2011. p.101-26;( 12 ) Castilho L, Pellegrino Júnior J, Reid ME. Fundamentos de imuno-hematologia. São Paulo: Atheneu; 2015. Sistemas de grupos sanguíneos com base em proteínas de passagem única. p. 45-15.( 13 )
Antigens of the Kell system are sensitive to treatment with mixture of trypsin and alpha-chymotrypsin. + antigens with intensified reactivity; 0: antigens that suffer no interference; - sensitive antigens, that can be destroyed; -/0 some antigens are destroyed or suffer no interference.
DTT: dithiothreitol; W: weak antigens.