Table 2. Performance assessment of three different presentations of papain for use in immunohematology in incubation time standardization tests.
| Type of treatment | Intensity of reaction | Score | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Time | ||||||||||
| 3’ | 5’ | 7’ | 10’ | 3’ | 5’ | 7’ | 10’ | |||
| Without treatment | 1+/2+ | 5/8 | ||||||||
| PAP1 | 3+ | 3+* | 2+/3+ | 2+/3+ | 10 | 10 | 8/10 | 8/10 | ||
| PAP2 | 3+ | 3+ | 3+/4+* | 2+/3+ | 10 | 10 | 10/12 | 8/10 | ||
| PAP3 | 4+ | 12 | ||||||||
Intensity of agglutination classified as crosses and score.
*
Most solid button compared to others (better time for sample). PAP1: American Association of Blood Banks protocol; PAP2: French protocol (three aliquots with activation of enzyme at the time of use); PAP3: commercial Bio-Rad™.