Fig. 4.

H₂O₂ upregulates immediate early genes, but not second-phase gene products. (A), (C) and (E) Cardiomyocytes were exposed to 0.2 mM H₂O₂ for 2 h in the absence or presence of 20 μM cycloheximide (CHX). (B), (D) and (F) Cardiomyocytes were exposed to 0.3 mM or 1 mM H₂O₂ for the times indicated. mRNA expression was measured by qPCR. Results are means ± SEM (n = 4–6 independent myocyte preparations). (A), (C) and (E) *p < .05, **p < .01 and ***p < .001 relative to unstimulated controls, # p < .05 relative to H₂O₂ alone (one-way ANOVA with Tukey post-test). (B), (D) and (F) *p < .05, **p < .01 and ***p < .001 relative to unstimulated controls, # p < .05 relative to 0.3 mM H₂O₂ (one-way ANOVA with Tukey post-test).