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. Author manuscript; available in PMC: 2019 May 2.
Published in final edited form as: ACS Infect Dis. 2018 Nov 26;5(1):79–89. doi: 10.1021/acsinfecdis.8b00199

Figure 4.

Figure 4.

Tetradecameric assembly and thermal stability of ClpP1 and ClpP2 homomeric complexes. (A) size exclusion chromatograph of purified recombinant ClpP1 and ClpP2 following Ni-affinity purification. B. subtilis ClpP (BsClpP) is included for comparison. (B) time-dependent oligomerization of ClpP2. An aliquot of ClpP2 was injected onto a S300 SEC (blue, solid) and again 14 days later (blue, dashed). (C and D) SYPRO Orange TSA of tetradecameric ClpP1 (C) and ClpP2 (D) in the presence and absence of varying concentrations of an ADEP activator.