Table 1.
Physical parameters of synthesized particles: size, the parameter characterizing poly-dispersity in size (PID), excitation/emission fluorescent maxima, fluorescent brightness, the zeta potential, and the number of folate molecules per single nanoparticle. For commercially available dyes were used to demonstrate the assembly of CA-dots fluorescent in the entire UV-VIS-NIR spectrum. Stilbene 420 (blue), Rhodamine 560 (green), Tracer Yellow (yellow), Methylene Blue (red/NIR) were used for encapsulation. Data for particles covered with PEG and PEG+folate are shown. Brightness relative to quantum dots was calculated for 1 QD450 41 (brightness 1 × 105 M−1 cm−1), 2 QD525 42 (brightness 1.3 × 105 M−1 cm−1), 3 QD585 42 (brightness 3.05 × 105 M−1 cm−1), QD705 43 (brightness 1.2 × 106 M−1 cm−1 ).
CA-dot | Size, Mn (nm) | PDI | Ex/Em (nm/nm) | Brightness (MBTU) / M cm−1 | Brightness relative to QD (times) | Zeta- Potential (mV) | Folates per particle |
---|---|---|---|---|---|---|---|
CA-SB-PEG | 61 | 0.22 | 400/445 | 89 ± 4 / (5.8±0.3) × 106 | 58 1 | −8.0±1 | N/A |
CA-SB-PEG-FA | 50 | 0.17 | 400/445 | 529 ± 42 / (3.4 ±0.3) ×107 | 340 1 | −11±1 | 870±220 |
CAGREEN-PEG | 77 | 0.13 | 495/525 | 870 ± 71 /(6.4 ±0.5) ×107 | 640 2 | −17±2 | N/A |
CA- GREEN - PEG-FA | 68 | 0.14 | 495/525 | 781 ± 49 / (5.8 ±0.4) × 107 | 580 2 | −15±3 | 910±200 |
CA-YEL- PEG | 60 | 0.15 | 450/560 | 159 ± 13 / (4.3±04) × 106 | 43 3 | −3±2 | N/A |
CA- YEL - PEG-FA | 87 | 0.11 | 450/560 | 649 ± 92 / (1.8 ±0.3) × 107 | 180 3 | −7±1 | 1110±180 |
CA- RED/NIR- PEG | 77 | 0.17 | 665/680 | 2554 ± 268 / (1.3 ±0.1) × 108 | 1300 4 | −19±1 | N/A |
CA- RED/NIR - PEG-FA | 63 | 0.20 | 665/680 | 318 ± 38 / (1.6 ±0.2) × 107 | 160 4 | −12±2 | 930±290 |