Fig 1. Representative autoradiographs of SP-PCR for progenitor allele estimation (ePAL) in four DM1 patients using two different DNA sources (left blood and right saliva).
The lower boundary of the allele distribution in each tissue was used to estimate the PAL. The bottom arrowhead indicates the PAL estimated in blood. In patients with blood ePAL < 150 CTG repeats (CR317 and CR145), the estimation of PAL using saliva was about the same, but in patients with an ePAL > 150 CTG repeats (CR333 and CR183) the ePAL measured in saliva was larger than in blood. The top arrowhead indicates the modal allele length for each tissue. For each sample, we indicate the ePAL measured in blood (ePAL), the age at sampling (Ages) and the age of onset (Ageo). The molecular weight marker sizes are shown converted to CTG repeat numbers.