Table 2. Quantitative FACS analysis of ATP1B2 patch I mutants.
Mean fluorescence intensity (MFI) from total cell population, calibrated against non-mutant ATP1B2. Given is the mean +/- SD of 3 independent experiments. Neg. control: untransfected cells.
| X-fold mutant type | ATP1B2 patch I variant |
MFI (% of non-mutant ATP1B2) |
|
|---|---|---|---|
| Per each ATP1B2 patch I variant | Per ATP1B2 patch I x-fold mutant type |
||
| - | Neg. control | 32.8 +/- 6.7 | 32.8 +/- 6.7 |
| Not mutated | ATP1B2 | 100 +/- 0.0 | 100 +/- 0.0 |
| Single mutants | Patch I region 1 | 187.9 +/- 129.6 | 95.1 +/- 53.0 |
| Patch I region 2 | 70.2 +/-22.2 | ||
| Patch I region 3 | 62.7 +/- 20.1 | ||
| Patch I region 4 | 60.8 +/- 29.3 | ||
| Double mutants | Patch I region 1+4 | 35.2 +/- 14.4 | 43.7 +/- 7.4 |
| Patch I region 2+4 | 48.8 +/- 14.9 | ||
| Patch I region 3+4 | 47.1 +/-10.6 | ||
| Triple mutants | Patch I region 1+2+3 | 34.2 +/- 11.7 | 36.7 +/- 4.4 |
| Patch I region 1+2+4 | 37.1 +/- 9.7 | ||
| Patch I region 1+3+4 | 42.8 +/- 25.8 | ||
| Patch I region 2+3+4 | 32.7 +/- 13.7 | ||
| Quadruple mutant | Patch I region 1+2+3+4 | 31.9 +/- 7.0 | 31.9 +/- 7.0 |