Fig 7. RyhB induces gentamicin resistance by inhibiting Fe-S clusters maturation.
The Δisc (A) and the Δsuf (B) strains containing or not ryhB were grown with (light grey bar) or without (dark grey bars) gentamicin (5 μg/mL) for 3 h in LB (left panels) or in LB with DIP (250 μM) (right panels). After that, cells were diluted in PBS and spotted on LB agar plates. c.f.u. and Log10 of surviving bacteria numbers were determined. Error bars represent the standard deviation of three independent experiments. Statistical analyses were performed with Student’s T-test: *p < 0,05; ***p < 0,001; N.S.: Not significant.