Table 2.
Reagents used for OMIP-053
| SPECIFICITY | CLONE | FLUOROCHROME | PURPOSE |
|---|---|---|---|
| Dead cells | N/A (dye) | Fixable blue | Viability |
| CD8 | SK1 | Brilliant violet 650™ | Exclusion |
| CD3 | SK7 | PerCP/Cy5.5 | Lineage |
| CD4 | SK3 | Brilliant violet 510™ | Lineage |
| CD127 | A019D5 | Brilliant violet 421™ | Treg identification (TDP I), sorting |
| CD25 | M-A251 | PE/dazzle™ 594 | Treg identification (TDP I and II), sorting |
| CD45RA | HI100 | Brilliant violet 711™ | Treg identification (TDP II), Treg activation status; |
| Naïve/memory classification | |||
| CCR7 | G043H7 | PE | Naïve/memory classification |
| HLA-DR | L243 | FITC | Activation |
| OPEN | OPEN | AF700 | Open |
| FoxP3 (ATREG PANEL) | 236A/E7 | APC | Treg identification (TDP I–III) |
| HELIOS (ATREG PANEL) | 22F6 | PE/Cy7 | Treg identification (TDP III) |
| Ki-67 (ATREG PANEL) | B56 | BUV395 | Proliferation |
| CD38 (BTREG PANEL) | HB7 | BUV395 | Activation |
| CD39 (BTREG PANEL) | A1 | APC/Fire™ 750 | Suppressive capacity and stability under inflammatory conditions |
| CD226 (DNAM-1) (BTREG PANEL) | HB7 | PE/Cy7 | Exclusion of functionally unstable Treg for in vitro expansion; tTreg identification |
| TIGIT (BTREG PANEL) | MBSA43 | APC | tTreg identification |