LN229 cells were transiently transfected with miRNA negative control (NC) or miR-4516 mimics, respectively. U87MG cells were transiently transfected with NC or miR-4516 inhibitor. Cell proliferation was measured at the different time points. n=5, **P<0.01, compared with controls (A-B). Colony formation assay was performed after NC, miR-4516 mimics, or miR-4516 inhibitor transfection (C-F). n=3, **P<0.01. The transwell invasion assay was conducted and numbers of invaded cells were quantified (G-J). n=3, **P<0.01.