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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Oncogene. 2018 Dec 17;38(16):2923–2936. doi: 10.1038/s41388-018-0601-9

Figure 5. miR-4516 regulates the Hippo pathway by targeting PTPN14.

Figure 5

(A-C) U87MG, LN18, and GBM30 cells were transfected with either control (pcDNA3) or pcDNA3/PTPN14 plasmid. Forty-eight hours post-transfection, western blotting was performed to determine the protein expression of PTPN14, pYAP, YAP, and GAPDH using respective antibodies. The intensity of each band was quantified using ImageJ and normalized to GAPDH and then to pcDNA3-transfected cells. (D-F) U87MG, LN18, and GBM30 cells were transiently transfected with either miRNA negative control (NC) or miR-4516 mimic. After 48 hours, western blotting was performed to determine the protein level of PTPN14, pYAP, YAP, and GAPDH using respective antibodies. The intensity of each band was quantified using ImageJ and normalized to GAPDH and then to NC-transfected cells.