Figure 6. One WT subunit in ASIC1a trimer is sufficient to induce SSD.

(A) Current traces of four trimers containing three wild-type subunits (WT-WT-WT), one (WT-Q276G-WT) or two (WT-Q276G-Q276G) mutant subunits elicited by pH 6.5 exhibit SSD but not channels with three (Q276G-Q276G-Q276G). WT-Q276G-Q276G trimers exhibit a small component of current resistant to desensitization. (B) pH response to SSD of the four trimers shown in (A).

Figure 6—figure supplement 1. Schematic and expression of trimers containing wild type or mutant monomers (Q276G).

(A) Cartoon representation of hASIC1a trimers constructed by ligation of three subunits using unique restriction sites. Each subunit has the indicated tag at the carboxyteminus. (B) Western blot analysis of the four trimers using anti-Flag, anti-V5 and anti-HA monoclonal antibodies. WT monomer (~68 kDa) detected with anti-HA monoclonal. The upper band ~130 kDa corresponds to aggregated subunits owing to overloading the lane with protein.