Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Anti-V5 HRP Mouse monoclonal	ThermoFisher Scientific	46–0708	Dilution 1:2000
Antibody	Anti FLAG M2 HRP Mouse monoclonal	Sigma	A8592-.2MG	Dilution 1:2000
Antibody	HA-probe (F-7) Mouse monoclonal	Santa Cruz Biotechnology	sc-7392	Dilution 1:2000
Antibody	Mouse IgG Secondary Antibody HRP conjugate	ThermoFisher Scientific	A16072	Dilution 1:10000
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1-hASIC1a	PMID: 23048040		Cloned from HEK293T
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1-ratASIC2a	PMID: 11382806		Cloned from rat dorsal root ganglia
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1-ratASIC3	PMID: 11382806
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1- hASIC1aHA-hAS IC1aV5-hASIC1aFl	This paper		Expression plasmid wt hASICa trimer
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1- hASIC1aQ276GHA-hASIC1aQ276GV5-hASIC1aQ276GFl	This paper		Expression plasmid hASIC1aQ276G trimer
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1-hASIC1aHA-hASICQ276GV5-hASIC1aFl	This paper		Expression plasmid one mutant subunit Q276G
Recombinant DNA reagent (DNA plasmid)	pcDNA3.1-hASIC1aHA-hASICQ276GV5-hASIC1aQ276GFl	This paper		Expression plasmid two mutant subunits Q276G
Peptide,	Psalmotoxin-1	Alomone labs	STP-200	five nM
Commercial assay or kit	QuickChange Site-Directed Mutagenesis Kit	Agilent	200523	Mutagenesis
Commercial assay or kit	mMESSAGEmMACHINE T7	ThermoFisher Scientific	AM1344	Synthesis of cRNA
Commercial assay or kit	Pierce BCA Protein Assay Kit	ThermoFisher Scientific	CN: 23227	Protein quantification
Chemical compound, drug	Spermine	SIGMA-ALDRICH	S3256-1G	0.25 mM
Chemical compound, drug	Tris(2-carboxyethyl)phosphine hydrochloride	SIGMA-ALDRICH	C4706	5 mM
Software, algorithm	PyMOL		RRID:SCR_000305
Software, algorithm	KaleidaGraph		RRID:SCR_014980